rhil 21 (R&D Systems)
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rhil 21
Rhil 21, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 49 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rhil+21/Recombinant+Human+IL-21+Protein/pmc13089343-228-29-32
Average 94 stars, based on 49 article reviews
Rhil 21, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 49 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rhil+21/Recombinant+Human+IL-21+Protein/pmc13089343-228-29-32
Average 94 stars, based on 49 article reviews
rhil 21 - by Bioz Stars,
2026-09
94/100 stars
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Immunopeptidomics:Article Title: Healthy donor T cell receptors expand functional neoantigen recognition beyond patient vaccination Article Snippet: For patients, pan CD8 + T cells were isolated using the CD8 + T cell Isolation Kit (Miltenyi Biotec, #130-096-495). .. Matured MoDCs were cocultured with the respective CD8 + T cells at a 1:4 ratio (MoDC:CD8 + T cell) in RPMI 1640 with 10% human serum (HS), 1% PS, Biomarker Discovery:Article Title: Healthy donor T cell receptors expand functional neoantigen recognition beyond patient vaccination Article Snippet: For patients, pan CD8 + T cells were isolated using the CD8 + T cell Isolation Kit (Miltenyi Biotec, #130-096-495). .. Matured MoDCs were cocultured with the respective CD8 + T cells at a 1:4 ratio (MoDC:CD8 + T cell) in RPMI 1640 with 10% human serum (HS), 1% PS, Recombinant:Article Title: The dysregulated IL-23/T H 17 axis in endometriosis pathophysiology Article Snippet: Activation consisted of 10μg/mL plate-bound anti-human CD3 antibody (300437) and 1μg/mL soluble anti-human CD28 antibody (302902) purchased from BioLegend, USA. .. Based on previously published protocols – , the T H 17 cocktail consisted of 5ng/mL recombinant human TGF-β1 (rhTGF-β1; 240-B-002), 12.5ng/mL rhIL-1β (201-LB-005), 25ng/mL rhIL-6 (206-IL-010), 25ng/mL Transfection:Article Title: Combining computational target prioritization and a B cell maturation assay for target evaluation studies in systemic lupus erythematosus. Article Snippet: Background and Purpose: Systemic lupus erythematosus (SLE) is a systemic autoimmune disease involving production of autoantibodies by B cells.. This study aimed at identifying novel drug targets using a computational algorithm to select targets and thereafter validate the top ranked 11 targets by siRNA knockdown in a primary B cell maturation assay.. Experimental Approach: The top 1 % genes (~150 genes) from SLE genome-wide association studies were ranked by Priority index (Pi), a computational tool integrating genomic and network information to prioritize diseaserelevant genes. Suspension:Article Title: Combining computational target prioritization and a B cell maturation assay for target evaluation studies in systemic lupus erythematosus. Article Snippet: Background and Purpose: Systemic lupus erythematosus (SLE) is a systemic autoimmune disease involving production of autoantibodies by B cells.. This study aimed at identifying novel drug targets using a computational algorithm to select targets and thereafter validate the top ranked 11 targets by siRNA knockdown in a primary B cell maturation assay.. Experimental Approach: The top 1 % genes (~150 genes) from SLE genome-wide association studies were ranked by Priority index (Pi), a computational tool integrating genomic and network information to prioritize diseaserelevant genes. Concentration Assay:Article Title: Combining computational target prioritization and a B cell maturation assay for target evaluation studies in systemic lupus erythematosus. Article Snippet: Background and Purpose: Systemic lupus erythematosus (SLE) is a systemic autoimmune disease involving production of autoantibodies by B cells.. This study aimed at identifying novel drug targets using a computational algorithm to select targets and thereafter validate the top ranked 11 targets by siRNA knockdown in a primary B cell maturation assay.. Experimental Approach: The top 1 % genes (~150 genes) from SLE genome-wide association studies were ranked by Priority index (Pi), a computational tool integrating genomic and network information to prioritize diseaserelevant genes. Article Title: Identifying novel B-cell targets for chronic inflammatory autoimmune disease by screening of chemical probes in a patient-derived cell assay. Article Snippet: A final volume of 200 l/well were seeded in 96 well round-bottom plates (Sarstedt). .. Probes and compounds diluted to 1M (1:10000) or 0,1M (1:100000) in DMSO, or only DMSO (0,01%, v/v) were added and plates were incubated 30 min at 37° C. Soluble rhCD40L (final concentration 500 ng/ml, R&D Systems), Cell Culture:Article Title: Single-cell multiomics reveal the scale of multilayered adaptations enabling CLL relapse during venetoclax therapy Article Snippet: Cryopreserved patient samples were thawed and labelled with 1 μM Cell Trace Violet (ThermoFisher) as described previously6. .. Cells were cultured on mouse embryonic fibroblast cells stably expressing the human CD40 ligand in the presence of Article Title: Novel CRISPR-Associated Gene-Editing Systems Discovered in Metagenomic Samples Enable Efficient and Specific Genome Engineering. Article Snippet: Primary human NK cell culture and nucleofection Cryopreserved human peripheral blood CD56+ cells were purchased from StemCell Technologies (StemCell No. 70037) and expanded using the Cloudz Human NK Cell Expansion kit (R&D Systems No. CLD004) according to the manufacturer’s protocol. .. Upon thaw, NK cells were seeded in T25 flasks with Cloudz anti-CD2- and anti-NKp46-coated microspheres and cultured in ExCellerate human NK cell expansion media (R&D Systems No. CCM032) supplemented with 10% FBS and varying concentrations of rhIL-2, rhIL-12, rhIL-18, and Article Title: Deficiency of base excision repair enzyme NEIL3 drives increased predisposition to autoimmunity Article Snippet: .. PBMCs were cultured with anti-CD40 (5 μg/ml; 626; Santa Cruz Biotechnology Inc.) with or without rhIL-4 (5ng/ml; R&D Systems) or Stable Transfection:Article Title: Single-cell multiomics reveal the scale of multilayered adaptations enabling CLL relapse during venetoclax therapy Article Snippet: Cryopreserved patient samples were thawed and labelled with 1 μM Cell Trace Violet (ThermoFisher) as described previously6. .. Cells were cultured on mouse embryonic fibroblast cells stably expressing the human CD40 ligand in the presence of Expressing:Article Title: Single-cell multiomics reveal the scale of multilayered adaptations enabling CLL relapse during venetoclax therapy Article Snippet: Cryopreserved patient samples were thawed and labelled with 1 μM Cell Trace Violet (ThermoFisher) as described previously6. .. Cells were cultured on mouse embryonic fibroblast cells stably expressing the human CD40 ligand in the presence of Incubation:Article Title: Identifying novel B-cell targets for chronic inflammatory autoimmune disease by screening of chemical probes in a patient-derived cell assay. Article Snippet: A final volume of 200 l/well were seeded in 96 well round-bottom plates (Sarstedt). .. Probes and compounds diluted to 1M (1:10000) or 0,1M (1:100000) in DMSO, or only DMSO (0,01%, v/v) were added and plates were incubated 30 min at 37° C. Soluble rhCD40L (final concentration 500 ng/ml, R&D Systems), |